A DNA library is a collection of DNA fragments that have been cloned into a vector, such as a plasmid or a phage. These libraries can be used for a variety of purposes, such as studying gene expression, identifying mutations, or identifying new genes.
There are two main types of DNA libraries: genomic libraries and cDNA libraries. Genomic libraries contain fragments of DNA from an entire genome, while cDNA libraries contain fragments of DNA made from the mRNA of a specific tissue or cell type.
DNA libraries are created by cutting DNA into fragments using restriction enzymes, and then cloning those fragments into a vector. The library can then be screened for specific genes or sequences using hybridization probes or PCR amplification.
DNA libraries have been instrumental in the development of many biotechnologies, including gene therapy, genetic engineering, and DNA sequencing. They are also used extensively in research to understand the function and regulation of genes.
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